摘要：目的：探討升清中藥抑制飲食誘導肥胖(DIO)大鼠肥胖及瘦素抵抗的作用機制。方法取 Wistar大鼠,制作DIO大鼠40只和DIO抵抗(DIO-R)大鼠10只。DIO大鼠分為模型組、西布曲明組、健脾理氣組、升清組,每日分別以生理鹽水、西布曲明、健脾理氣藥物(陳皮、木香、枳實)、升清藥物(柴胡、升麻、葛根)灌胃,空白組與DIO-R組予生理鹽水,灌胃16周。測定干預前后體質量,檢測干預后血清神經肽Y(NPY)、瘦素和脂肪瘦素、細胞因子信號傳導抑制因子3(SOCS-3)。結果與DIO-R組比較,模型組體質量增加、NPY升高(P＜0.01),血清和脂肪瘦素降低(P＜0.05),SOCS-3升高(P＜0.05)。藥物干預后,各藥物組體質量有所下降。健脾理氣組脂肪瘦素升高(P＜0.05)；升清組在升高瘦素、降低體質量和 NPY、SOCS-3水平方面,優于西布曲明組和健脾理氣組,差異有統計學意義(P＜0.05,P＜0.01)。結論升清藥物能減輕肥胖,抑制瘦素抵抗。%Objective To observe the effects of Shengqing Chinese herbs on the obesity and leptin resistance of diet-induced obesity (DIO) rats.Methods Wistar rats were produced a rat model including of 40 DIO rats and 10 DIO-R rats. The DIO rats were divided into DIO model group, sibutramine group, Jianpi Liqi group and Shengqing group, which were respectively by gavage of saline, sibutramine, Jianpi Liqi herbs (Citri reticulatae pericarpium, Aucklandiae radix, Aurantii fructus immaturus), Shengqing herbs (Bupleuri radix, Cimicifugae rhizoma, Puerariae lobatae radix), blank control group and DIO-R group were by gavage of saline. Their weight, were examined after 16 weeks gavage. Leptin and neuropeptide Y in the serum were examined. Leptin, TNF-α, and suppressors of cytokine signaling-3 (SOCS-3) in the homogenized fat were examined. Results Compared with the DIO-R group, DIO model group's body weight, NPY significantly increased (P<0.01);leptin in the serum and homogenized fat decreased (P<0.05);SOCS-3 increased (P<0.05). After drug intervention, weight of each drug group declined. Compared with the DIO model group, Jianpi Liqi group's leptin in the homogenized fat increased (P<0.05). Shengqing group's leptin in homogenized fat increased, body weight reduced, NPY and SOCS-3 levels decreased, and better than sibutramine group and Jianpi Liqi group (P<0.05,P<0.01).Conclusion Shengqing herbs can reduce obesity and inhibit leptin resistance.