摘要：為監測茶鮮葉中有機磷農藥殘留污染狀況,應用超聲波提取、分散固相萃取凈化、氣相色譜檢測,建立了一種簡單、快速測定茶鮮葉中7種有機磷農藥(敵敵畏、樂果、毒死蜱、甲基對硫磷、馬拉硫磷、殺螟硫磷和三唑磷)殘留的分析方法。樣品采用φ(乙酸)=1%的乙腈溶液超聲提取,乙二胺-N-丙基硅烷、十八烷基硅烷鍵合硅膠和石墨化炭黑混合吸附劑分散萃取凈化, DB-1701毛細管柱程序升溫分離,火焰光度檢測器測定,基質外標法定量。結果表明,在0.01~4.00 mg·L-1范圍內,7種有機磷農藥的峰面積與其濃度呈良好的線性關系,相關系數均大于0.998,方法的檢出限為0.0034~0.0136 mg·kg-1,定量限為0.012~0.046 mg·kg-1,加標回收率為77.7%~98.2%,相對標準偏差( n=5)為2.4%~8.7%。該方法操作簡單、結果可靠、溶劑用量少,對檢測條件要求低,適用于茶鮮葉中7種有機磷農藥殘留的檢測。%To investigate the pollution of organophosphorus pesticides in fresh tea leaves, a simple and rapid analytical method was established for simultaneous determination of seven organophosphorus pesti-cides ( dichlorvos, dimethoate, chlorpyrifos, methyl parathion, malathion, fenitrothion, and triazophos) in fresh tea leaves by ultrasound assisted extraction and dispersive solid phase extraction ( DSPE) coupled with gas chromatography. The samples were ultrasonically extracted with acetonitrile ( containing 1% ace-tic acid) . Subsequently, the extract was cleaned-up by using primary secondary amine, octadecylsilane bonded silica and graphitized carbon black as adsorbents according to the method of DSPE. Then, the residues were determined by a heated DB-1701 capillary column with flame photometric detector, and quantified using the matrix-matched external standard method. Good linearities between concentrations and peak areas were achieved in the concentration range of 0. 01 -4. 00 mg·L-1 for target pesticides, with correlation coefficients higher than 0. 998 . The average recoveries of target pesticides in fresh tea leaves were 77. 7% -98. 2% with relative standard deviations ( n =5 ) ranging from 2. 4% to 8. 7%. The limits of detection for target organophosphorus pesticides were 0. 003 4 -0. 013 6 mg·kg-1 and the limits of quantification were 0. 012-0. 046 mg·kg-1 . The method was simple, accurate, solvent saving and without special instrument making it suitable for simultaneous determination of seven organophospho-rus pesticides in fresh tea leaves.
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